Scale-Up of Native β-Lactoglobulin Affinity Separation Process
نویسندگان
چکیده
منابع مشابه
pH-Dependent aggregation and disaggregation of native β-lactoglobulin in low salt.
The aggregation of β-lactoglobulin (BLG) near its isoelectric point was studied as a function of ionic strength and pH. We compared the behavior of native BLG with those of its two isoforms, BLG-A and BLG-B, and with that of a protein with a very similar pI, bovine serum albumin (BSA). Rates of aggregation were obtained through a highly precise and convenient pH/turbidimetric titration that mea...
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In ideal proteins, only native interactions are stabilized step-by-step in a smooth funnel-like energy landscape. In real proteins, however, the transient formation of non-native structures is frequently observed. In this review, the transient formation of non-native structures is described using the non-native helix formation during the folding of β-lactoglobulin as a prominent example. Althou...
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Interfacial properties of native β-lactoglobulin monomers and their heat-induced fibers, of two different lengths, were investigated at pH 2, through surface tension measurements at water-air and water-oil interfaces and interfacial shear rheology at the water-oil interface. The applied heat treatment generates a mixed system of fibers with unconverted monomers and hydrolyzed peptides. The surf...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2002
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(02)74236-7